Arsenal midfielder Jack Wilshere
will not return to the England squad for Tuesday's international
friendly against Italy at Wembley.
He was hoping to win his first England cap since the last-16 exit to Iceland at Euro 2016.
Liverpool's Joe Gomez will stay with the squad for further assessment on the injury he sustained against the Dutch.
The 20-year-old defender, who started on the left side of a back three, was substituted in the 10th minute of the friendly.
Southgate had been hopeful Wilshere, who was recalled to the England squad after a sustained run in the Arsenal side, would be able to play some part against Italy.
Wilshere will remain with the Gunners, who host Stoke on 1 April in their next game.
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